Methods, Protocols and Techniques

General

• Biological Research Protocol Books - List of books describing methods and techniques in biochemistry, molecular biology and other life sciences.
• BioVisa.net Protocols - Searchable collection of protocols in biology and biochemistry.
• Cell Biology Laboratory Manual
• Cold Spring Harbor Protocols - An online interactive biology laboratory protocols database.
• Course in the Protection of Human Research Subjects
• Current Protocols - Online resource for publications on laboratory methods in genetics, protein science, immunology, cell biology, toxicology, biochemistry, molecular biology. (John Wiley and Sons, Inc., Publisher)
• Cytoskeleton Information Resource
• Flow Cytometry on the Web - List of resources devoted to flow cytometry.
• GFP - Green Fluorescent Protein Applications - A site devoted to applications of GFP with links to numerous resources. (Wallace Marshall)
• Histology and Immunohistochemistry Protocol Database (IHC World)
• Immunoportal.com: Immunohistochemistry - In Situ Hybridization - Useful site with information and protocols.
• International Society For Molecular and Cell Biology Protocols and Researches
• The Joy of Cloning
• The Medical Biochemistry Page: Molecular Tools of Medicine - An introduction to the methods of molecular biology. (Michael W. King, Terre Haute Center for Medical Education, Indiana University School of Medicine)
• microarrays.org - "Microarrays.org is a public source for microarray protocols and software." (DeRisi Lab, University of California, San Francisco.)
• MIT Biology Hypertextbook: Recombinant DNA (Beginner's Level)
• Molecular Biology Notebook (Rothamsted Research)
• Molecular Biology Protocols on the World Wide Web
• Protocol Online - Large directory of protocols in molecular biology, cell biology and immunology.
• Tissue Culture Methods (Julie B. Wolf, University of Maryland, Baltimore County)
• westernblotting.org - Western blot protocols and troubleshooting.
• Yeast Two-Hybrid System - Links to two-hybrid system and other yeast protocols (Tritech Research)

See also the Cell Biology Laboratories page (labs providing protocols are indicated) and the Scientific Databases and Tools page.

Discussion Groups

• BioChemWeb.org Discussion Board - Discussions and questions/answers for scientists and students.

• BioTechniques Molecular Biology Techniques Forums
• IHC World Forum
• Protocol Online: BioForum: Methods Discussion
• Usenet newsgroup archives from BIOSCI/bionet (can also be accessed through Google Groups: bionet):
  • ARRAYS/bionet.molbio.genearrays - Gene array and microarray technology.
  • AUTOSEQ/bionet.genome.autosequencing - Research and support on automated DNA sequencing.
  • BIOCHROM/bionet.genome.chromosomes - Mapping and sequencing of eukaryotic chromosomes.
  • GEN-LINK/bionet.molbio.gene-linkage - Discussions about genetic linkage analysis.
  • METHDS-REAGNTS/bionet.molbio.methds-reagnts - Methods and reagents in molecular biology.
  • RAPD/bionet.molbio.rapd - Research on Randomly Amplified Polymorphic DNA.
  • RECOM/bionet.molbio.recombination - Research on the recombination of DNA or RNA.

See also the Discussion Groups section of the General Biochemistry and Cell Biology page.