Fenteany Research Group

Enzyme assays

• JNK kinase assay

Actin in vitro

• Acetone powder preparation
• Actin extraction from acetone powder
• Chromatographic purification of actin
• Labeling of actin with pyrene iodoacetamide

Cells

• Immunostaining cells
• Staining cells for F-actin with fluorescently labeled phalloidin

Xenopus

• In situ hybridization

Misc. basic

• SDS-PAGE recipes
• Western blotting protocol